Mass Cytometry Reveals Distinct Platelet Subtypes in Healthy Subjects and Novel Alterations in Surface Glycoproteins in Glanzmann Thrombasthenia

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Evaluation of platelet surface glycoproteins in patients with Glanzmann thrombasthenia: Association with bleeding symptoms

BACKGROUND & OBJECTIVES Glanzmann thrombasthenia (GT) is a rare, inherited autosomal recessive disorder characterized by qualitative or quantitative deficiency of integrin αIIbβ3 [glycoprotein IIb (GPIIb)/IIIa, CD41/CD61] diagnosed by absent or reduced platelet aggregation to physiological agonists, namely, collagen, adenosine-di-phosphate, epinephrine and arachidonic acid. The objective of thi...

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Antibodies that bind to human platelet membrane glycoproteins llb and lIla were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their lowintensity light scatter profiles in whole blood or plateletrich plasma preparations. Identification of this cell population as platelets was verified by using plateletspecific antib...

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Antibodies that bind to human platelet membrane glycoproteins IIb and IIIa were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their low-intensity light scatter profiles in whole blood or platelet-rich plasma preparations. Identification of this cell population as platelets was verified by using platelet-specific an...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2018

ISSN: 2045-2322

DOI: 10.1038/s41598-018-28211-5